Introduction
Assessment of Neuronal Activity Using Microelectrode Array:
- The eCiphr®Neuro assay uses primary cultures of rat cortical neurons.
- Cyprotex’s neuronal assay uses high throughput microelectrode array (MEA) technology to monitor electrophysiological activity.
- Neurons grown on microelectrode arrays recapitulate many features of neurons in vivo, including spontaneous activity (spiking and bursting), plasticity, organisation and responsiveness to a wide range of neurotransmitters and pharmacological agonists/antagonists1.
- This technology provides a unique in vitro system for preclinical drug discovery, neurotoxicity assessment and disease modelling.
Protocol
eCiphr®Neuro Assay Protocol
Data
Data from Cyprotex's eCiphr®Neuro Assay
References
1) Robinette BL et al, (2011) Front Neuroeng 4; Article 1
2) Margineau DG and Wülfert E (1997) Br J Pharmacol 122; 1146-1150
3) Mackenzie L et al, (2002) Clin Neurophysiol 113(4); 586-596
4) Ono J et al, (1990) Funct Neurol 5(4); 345-352
5) Fuentealba J et al, (2011) Neuropharmacology 60; 453-459
6) Levy LM and Degnan AJ, (2013) Am J Neuroradiol 34(2); 259-265
7) Hwang DF and Noguchi T (2007) Adv Food Nutr Res 52; 141-236
8) Peña F and Tapia R (2000) Neuroscience 101(3); 547-561
9) Pulido OM (2008) Mar Drugs 6(2); 180-219
10) Hankir MK et al, (2012) Neuroimage 59(2); 968-978
11) Kehne JH et al, (1992) Br J Pharmacol 106(4); 910-916
12) MEA, Axion Biosystems
Downloads
- Cyprotex Mechanisms of Drug Induced Toxicity Guide >
- The Future of Microelectrode Array in Early Toxicology Assessment March 2020 >
- Cyprotex eCiphr®Neuro Factsheet >