Toxicological Gene Regulation - Custom Toxicology Service
Background Information
- Toxicogenomics is the application of transcription profiling to toxicology. It is often used to predict toxicity before functional damage is observed and is based on the assumption that compounds which elicit toxicity through similar mechanisms have particular signatures of gene expression.
- One example of where gene expression profiles have been successfully linked to toxicity is the assessment of oxidative stress-associated hepatotoxicity related to a single specific mechanism1,2.
- Real Time quantitative Reverse Transcription PCR (qRT-PCR) is one of the most sensitive techniques for mRNA detection and quantitation and is used to confirm results from microarray analysis.
- Cyprotex has purchased an Applied Biosystems 7900HT system and have the ability to monitor the expression of a wide range of different toxicologically relevant genes using qRT-PCR.
References
1) McMillian M et al. (2004) Biochem Pharmacol 67; 2141–2165
2) McMillian M et al. (2004) Biochem Pharmacol 68; 2249–2261
3) Blomme EAG et al. (2009) Toxicology Letters 186; 22–31
Cyprotex enables and enhances the prediction of human exposure, clinical efficacy and toxicological outcome of a drug or chemical. By combining quality data from robust in vitro methods with contemporary in silico technology, we add value, context and relevance to the ADME-Tox data supplied to our partners in the pharmaceutical or chemical industries.
