Protein Biochemistry

Within protein biochemistry, we recognize the unique needs and philosophies of each partner. As a team we strive to offer flexible, innovative, and efficient solutions acting as an extension of your lab, where scientists drive your projects with the motivation to be better than yesterday.

Evotec’s protein biochemistry team has a track record of success over 20 years across a range of diverse and complex target classes. We are equipped to support all your biochemistry needs from gene to protein and beyond into integrated drug discovery solutions.

• Membrane proteins (GPCRs, Ion channels) / Epigenetic complexes / Lipid Kinases and intrinsically disordered proteins
• Protein complexes (Protein-Protein interactions) / Protein-DNA/RNA complexes
• Rapid automated production to expedite the generation of a data rich package to inform large scale productions thereby reducing timelines and costs

Our team has the experience to engineer proteins for numerous applications supporting structural biology, hit identification, biocatalyst, biotherapeutics and beyond:

• Biocatalysis
  • Hight Throughput enzyme panel screening
  • Structural enablement 
  • Protein thermostabilizing
  • Fermentation scale-up and lyophilization
    
• Protein Biologics:
  • Protein biologics engineering supporting enzymes and fc-fusions
  • High-throughput automated platform to support complex biologics production (bs-Abs, ms-Abs) / Antigens / Fc-fusions
  • Bioreactor scale-up with continuous metabolic control with a BioProfile FLEX2
  • Stable and transient protein production from mg to gram
  • Endotoxin free antigen and biologics production
  • Stability optimization

To effectively drive your scientific projects, Evotec utilizes the most appropriate technology coupled with dedicated scientific experts. With access to a range of expression and purification technologies and access to multiple downstream biochemical, biophysical assay technologies, Evotec scientists will design, generate and analyze the reagent to ensure they fit for purpose. 

• Smart construct design through application of AI/ML tools, bioinformatics and structural modelling
• A broad range of expression systems including insect, mammalian, yeast, and bacterial with access to knockout and knock in technologies with our CRISPR team
• Protein labelling and conjugation (isotope, non natural amino acids, chemical modification, biotinylation and dyes)

Regardless of the target of interest Evotec has access to the enabling technologies required.

• Mass Spec Mass Spectrometry TOF and Orbitrap
• nano-DSF
• DLS and SLS
• SEC-MALS
• Biacore SPR and Creoptix wave GCI
• MST
• ITC
• Multiple plate readers supporting functional and biochemical activity assays
• Automated Automated analytical and florescent size exclusion chromatography
• AKTA purification systems including large scale AKTA pilot
• LabChip CE
• BioProfile FLEX2 metabolite analysis