3D Microtissue & Multilayer Models
High Content Imaging of 3D Microtissues
Introduction
- The environment created by a 3D culture model allows reconstitution of the natural cellular physiology by promoting the complex cell-cell and cell-matrix network communications found in vivo.
- For imaging purposes, microtissues are formed using round bottom ultra-low adhesion cell culture plates which promote aggregation of cells into functional 3D microtissues.
- Microtissues can be formed from single or co-cultured cell populations and their longevity permits use in long-term repeat dose toxicity studies to investigate any cumulative effect.
- The combination of biologically relevant in vitro 3D cell-based models with multiparametric HCS assays presents a viable screening strategy for the detection of novel therapeutics that cause toxicity early in drug development.
Protocol
High Content Imaging of 3D Microtissues
Data
Data from High Content Imaging of 3D Microtissues
Cyprotex enables and enhances the prediction of human exposure, clinical efficacy and toxicological outcome of a drug or chemical. By combining quality data from robust in vitro methods with contemporary in silico technology, we add value, context and relevance to the ADME-Tox data supplied to our partners in the pharmaceutical or chemical industries.
